Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CDK1 antibody [BLR085G] - BSA free (ab272070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR085G] to CDK1 - BSA free
Suitable for: IP, IHC-P, ICC
Reacts with: Human

Product name

Anti-CDK1 antibody [BLR085G] - BSA free
See all CDK1 primary antibodies
Description

Rabbit monoclonal [BLR085G] to CDK1 - BSA free
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CDK1 aa 1-50. The exact sequence is proprietary. NP_001777.1
Database link: P06493
Positive control
- IHC-P: Human breast carcinoma and prostate carcinoma tissue. ICC: MOLT4 cells. IP: HeLa whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc.

ab272070 is the carrier-free version of ab265590. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 98% Borate buffered saline
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR085G
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [BLR085G] - BSA free (ab272070)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CDK1 with ab265590 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265590).
Immunoprecipitation - Anti-CDK1 antibody [BLR085G] - BSA free (ab272070)

CDK1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265590 at 20 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab265590 at 1/1000 dilution.

Lane 1: ab265590 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265590).
Immunocytochemistry - Anti-CDK1 antibody [BLR085G] - BSA free (ab272070)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded molt4 cells labeling CDK1 with ab265590 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265590).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 antibody [BLR085G] - BSA free (ab272070)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling CDK1 with ab265590 at 1/100 dilution. Detection: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265590).
Anti-CDK1 antibody [BLR085G] - BSA free (ab272070)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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