Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free
See all CDK1 primary antibodies
Description

Rabbit monoclonal [EPR19546] to CDK1 (phospho T161) - BSA and Azide free
Host species

Rabbit
Specificity

This antibody also recognizes CDK2 (phospho T160) and CDK3 (phospho T160).

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab251327 is the carrier-free version of ab201008. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251327 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19546
Isotype

IgG
Research areas

Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with UV for 90 minutes whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28,34 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

This data was developed using ab201008, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Dot Blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.
Dot blot analysis of CDK1 (phospho T161) labeled with ab201008 at 1/1000 dilution. Lane 1: CDK1 (phospho T161) phospho peptide.
Lane 2: CDK1 non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
Dot Blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.
Dot blot analysis of CDK2 (phospho T160) labeled with ab201008 at 1/1000 dilution. Lane 1: CDK2 (phospho T160) phospho peptide.
Lane 2: CDK2 non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
Dot Blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.
Dot blot analysis of CDK3 (phospho T160) labeled with ab201008 at 1/1000 dilution. Lane 1: CDK3 (phospho T160) phospho peptide.
Lane 2: CDK3 non-phospho peptide. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa treated with UV for 90 minutes whole cell lysate
Lane 3 : HeLa treated with UV for 90 minutes then with alkaline phosphatase for 1-hour whole cell lysate
Lane 4 : HeLa treated with UV for 90 minutes then with alkaline phosphatase overnight whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?

Exposure time: 15 seconds

This data was developed using ab201008, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : C6 treated with alkaline phosphatase for 1 hour whole cell lysate
Lane 3 : C6 treated with alkaline phosphatase overnight whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28,34 kDa why is the actual band size different from the predicted?

Exposure time: 10 seconds

This data was developed using ab201008, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

All lanes : Anti-CDK1 (phospho T161) antibody [EPR19546] (ab201008) at 1/1000 dilution

Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : C6 (rat glial tumor cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

This data was developed using ab201008, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and weak cytoplasm staining of germinal center from human tonsil is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining of cancer cells from human cervix cancer is observed [PMID: 15623629]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling CDK1 (phospho T161) with ab201008 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus and cytoplasm staining of rat testis is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CDK1 (phospho T161) with ab201008 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing specific signal in M phase cells. The signal decreased after treatment with lambda protein phosphatase 31°C for 5 hours. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
Immunoprecipitation - Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)

This data was developed using ab201008, the same antibody clone in a different buffer formulation.

CDK1 (phospho T161) was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab201008 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201008 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa treated with 25J/m2 UV for 1 hour whole cell lysate 10 µg (Input).

Lane 2: ab201008 IP in HeLa treated with 25J/m2 UV for 1 hour whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201008 in HeLa treated with 25J/m2 UV for 1 hour whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-CDK1 (phospho T161) antibody [EPR19546] - BSA and Azide free (ab251327)