Anti-DDX21 antibody (ab244457)
Key features and details
- Rabbit polyclonal to DDX21
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DDX21 antibody
See all DDX21 primary antibodies -
Description
Rabbit polyclonal to DDX21 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human DDX21 aa 1-150.
Database link: Q9NR30 -
Positive control
- IHC-P: Human appendix tissue. WB: Caco-2, HeLa, RT4 and U-251 MG cell lysates. ICC/IF: U-251 MG cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-DDX21 antibody (ab244457)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for DDX21 (green) using ab244457 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 antibody (ab244457)Paraffin-embedded human appendix tissue stained for DDX21 using ab244457 at 1/200 dilution in immunohistochemical analysis. -
Western blot - Anti-DDX21 antibody (ab244457)All lanes : Anti-DDX21 antibody (ab244457) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 87 kDa
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Western blot - Anti-DDX21 antibody (ab244457)All lanes : Anti-DDX21 antibody (ab244457) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 87 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX21 antibody (ab244457)Paraffin-embedded human skeletal muscle tissue stained for DDX21 using ab244457 at 1/200 dilution in immunohistochemical analysis.
