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Human IL-28A ELISA Kit (ab238269)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 25.24 pg/ml
  • Range: 98.44 pg/ml - 6300 pg/ml
  • Sample type: Cell culture supernatant
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-28A ELISA Kit
    See all IL-28A kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HUVEC 8 2.8%

  • Sample type

    Cell culture supernatant

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    25.24 pg/ml

  • Range

    98.44 pg/ml - 6300 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 101 % - %

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-28A ELISA Kit (ab238269) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-28A protein in cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-28A with 25.24 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-28A Capture Antibody 1 x 600µl
    10X Human IL-28A Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IL-28A Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway.

  • Sequence similarities

    Belongs to the IL-28/IL-29 family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Cytokine ZCYTO20
    • IFN-lambda-2
    • IFNL2
    • IL-28A
    • IL28A
    • IL28A_HUMAN
    • Interferon lambda 2
    • Interferon lambda-2
    • Interleukin 28A
    • interleukin 28A (interferon, lambda 2)
    • Interleukin-28A
    • ZCYTO20
    see all

  • Database links

    Images

    • Other - Human IL-28A ELISA Kit (ab238269)
      Other - Human IL-28A ELISA Kit (ab238269)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human IL28A standard curve in Sample Diluent NS.
      Example of human IL28A standard curve in Sample Diluent NS.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native IL28A in unstimulated and stimulated HUVEC cell culture supernatant samples.
      Interpolated concentrations of native IL28A in unstimulated and stimulated HUVEC cell culture supernatant samples.

      HUVEC cells were stimulated by adding human IFN-a at 100 IU/mL for 20 hours, followed by the addition of Poly(I:C) at 30 µg/mL for 3 hours. The concentrations of IL28A were measured in duplicates, interpolated from the IL28A standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated HUVEC cell supernatant 100% and stimulated HUVEC cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). IL28A was not detectable in unstimulated HUVEC cell culture supernatant. The mean IL28A concentration was determined to be 545.5 pg/mL in stimulated HUVEC cell culture supernatant.

    • Sandwich ELISA - Human IL-28A ELISA Kit (ab238269)
      Sandwich ELISA - Human IL-28A ELISA Kit (ab238269)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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