Anti-BRD3 antibody (ab264294)
Key features and details
- Rabbit polyclonal to BRD3
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-BRD3 antibody
See all BRD3 primary antibodies -
Description
Rabbit polyclonal to BRD3 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BRD3 aa 676-726. The exact sequence is proprietary. NP_031397.1
Database link: Q15059 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-BRD3 antibody (ab264294)All lanes : Anti-BRD3 antibody (ab264294) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 80 kDa
Exposure time: 30 seconds4-8% SDS-PAGE.
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Immunoprecipitation - Anti-BRD3 antibody (ab264294)BRD3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264294 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264294 at 1 µg/ml.
Lane 1: ab264294 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 secs.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRD3 antibody (ab264294)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling BRD3 with ab264294 at 1/100 dilution. Detection: DAB.
