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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-PLK1 antibody (ab235193)

Price and availability

278 083 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-PLK1 antibody (ab235193)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Chicken polyclonal to PLK1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-PLK1 antibody
    See all PLK1 primary antibodies
  • Description

    Chicken polyclonal to PLK1
  • Host species

    Chicken
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T PLK1 overexpressing lysate; HEK-293T and K562 cell lysate. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgY fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Cell cycle
    • Cell division

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PLK1 antibody (ab235193)
    Immunocytochemistry/ Immunofluorescence - Anti-PLK1 antibody (ab235193)

    4% paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PLK1 (green) using ab235193 at 5 μg/ml in ICC/IF. Donkey Anti-Chicken IgG DyLight™ 488 Preadsorbed was used as the secondary antibody. Nuclear counterstain was DAPI.

    Expected localization: Intracellular.

    A) DAPI
    B) Primary + Secondary incubation
    C) Merge of A+B
    D) Secondary only

  • Western blot - Anti-PLK1 antibody (ab235193)
    Western blot - Anti-PLK1 antibody (ab235193)
    All lanes : Anti-PLK1 antibody (ab235193) at 1/500 dilution

    Lane 1 : Opal pre-stained ladder
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) PLK overexpressing lysate
    Lane 3 : HEK-293T cell lysate
    Lane 4 : Human testis lysate
    Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Secondary
    All lanes : Goat Anti-Chicken at 1/40000 dilution

    Predicted band size: 68 kDa
    Observed band size: 135, 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 7 seconds


    In some lysates 63 kDa band is present.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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