Anti-Cdc6 antibody - N-terminal (ab188423)
Key features and details
- Rabbit polyclonal to Cdc6 - N-terminal
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cdc6 antibody - N-terminal
See all Cdc6 primary antibodies -
Description
Rabbit polyclonal to Cdc6 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human Cdc6 aa 1-350 (N terminal). (Expressed in E. coli).
Database link: Q99741 -
Positive control
- HeLa whole cell lysate (ab150035); HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (ab188423)
Immunocytochemistry/ Immunofluorescence analysis of MEFs (mouse embryonic fibroblasts) from embryonic day 14.5 mouse embryos labeling Cdc6 with ab188423 at 1/200 dilution. The cells were fixed with 10 % formalin at room temperature (RT) for 10 min, permeabilized with ice-cold methanol on ice for 10 min, and treated with 2 % BSA/PBS at RT for 1h. Cells were incubated with Anti-Cdc6 antibody - N-terminal (ab188423) at 4°C overnight and then with Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody (1/1000) at RT for 1h. Chromosomal DNA was counterstained with 3.3 μM Hoechst 33342. Note that CDC6 abundantly localizes in nuclei of the cells at G1 and early S phase.
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Immunoprecipitation - Anti-Cdc6 antibody - N-terminal (ab188423)Immunoprecipitation analysis of HeLa whole cell lysate.
Lane 1: Control immunoprecipitation with non-immune IgG
Lane 2: Immunoprecipitates wtih ab188423
Lane 3: Input.
Western blot detection with ab188423 at 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 antibody - N-terminal (ab188423)Immunofluorescent analysis of HeLa cells in G1 or early S phase. Cells in mid to late G1phase or hydroxyurea-treated early S phase were fixed with formaldehyde directly or after extraction with Triton X-100 and then immunostained using ab188423 at 1/100 dilution or control non-immune antibody (Alexa;green). The samples were further treated with propidium iodide for DNA staining (PI; red).
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Western blot - Anti-Cdc6 antibody - N-terminal (ab188423)Anti-Cdc6 antibody - N-terminal (ab188423) at 1/1000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 63 kDa
