Antibodies, Proteins, Kits and Reagents for Life Science

251 280 ₸ Product size

100 µl 251 280 ₸

Order now and get it on Thursday March 04, 2021

Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54)
Suitable for: WB, ICC/IF, Dot blot
Reacts with: Human

Product name

Anti-Cdc6 (phospho S54) antibody [EPR759Y]
See all Cdc6 primary antibodies
Description

Rabbit monoclonal [EPR759Y] to Cdc6 (phospho S54)
Host species

Rabbit
Specificity

ab75809 recognises Cdc6 only when phosphorylated at Serine 54.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6
Positive control
- WB: Raji + FBS cell lysates. ICC: HeLa cells. Dot Blot: Cdc6 (pS54) phospho peptide.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR759Y
Isotype

IgG
Research areas

Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)

All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/5000 dilution

Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with Hydroxyurea (3mM, 20 h). Whole cell lysates.Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 63 kDa

Exposure time: 30 seconds

Blocking buffer and diluting buffer: 5% NFDM/TBST
Dot Blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)

Primary antibody dilution 1:1000 dilution. Secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051). Secondary ab dilution 1:100,000 dilution. Blocking buffer and concentration 5% NFDM/TBST. Diluting buffer and concentration 5% NFDM /TBST. Lane 1:Cdc6 (pS54) phospho peptide, Lane 2:Cdc6 non-phospho peptide. Exposure time 3 minutes
Immunocytochemistry/ Immunofluorescence - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)

Cell line 293T (Human embryonic kidney epithelial cell). Target AbID Ab75809 anti- Cdc6 (phospho S54)

ab150077 AlexaFluor®488 Goat anti-Rabbit secondary. Counterstain AbID Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Fixative 4% Paraformaldehyde. Permeabilisation 0.1% tritonX-100. Nuclear counter stain DAPI. Datasheet Comments Confocal image showing the expression was increased after treatment with Hydroxyurea (3mM 20hour), the signal was decreased after treatment with the Lambda Protein Phosphatase 31℃ for 2h. Target 1oAb dilution 1:100 2.4 μg/ml, Target 2ndry Ab dilution 1:1000 2 μg/ml, Counterstain Ab dilution 1:200 2.5 μg/ml
Western blot - Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)

All lanes : Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809) at 1/2000 dilution

Lane 1 : Raji cell lysate
Lane 2 : Raji +
FBS cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa
Observed band size: 63 kDa
Anti-Cdc6 (phospho S54) antibody [EPR759Y] (ab75809)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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