Anti-Sp7 / Osterix antibody (ab22552)
Key features and details
- Rabbit polyclonal to Sp7 / Osterix
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Sp7 / Osterix antibody
See all Sp7 / Osterix primary antibodies -
Description
Rabbit polyclonal to Sp7 / Osterix -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatHuman
-
Immunogen
Synthetic peptide corresponding to Mouse Sp7/ Osterix aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab24390) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
ChIP Related Products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species IHC-P MouseRatHumanApplication Abreviews Notes IHC-P (11) 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.Notes IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.Target
-
Function
Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences. -
Tissue specificity
Restricted to bone-derived cell. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 121340 Human
- Entrez Gene: 170574 Mouse
- Entrez Gene: 300260 Rat
- Omim: 606633 Human
- SwissProt: Q8TDD2 Human
- SwissProt: Q8VI67 Mouse
- Unigene: 209402 Human
- Unigene: 263284 Mouse
see all -
Alternative names
- MGC126598 antibody
- Osterix antibody
- Osx antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)This image is courtesy of an anonymous collaborator
ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)This image is courtesy of Jérôme AMIAUD, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives. We thank them for their important contribution to the validation of ab22552.Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)Image is courtesy of Dr Faye Dohertyab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)Image courtesy of Human Protein AtlasImage courtesy of Human Protein Atlas
ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552)This image is courtesy of an anonymous AbreviewThe image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (223)
ab22552 has been referenced in 223 publications.
- Ge X et al. Parathyroid hormone enhances the osteo/odontogenic differentiation of dental pulp stem cells via ERK and P38 MAPK pathways. J Cell Physiol 235:1209-1221 (2020). PubMed: 31276209
- Zhu J et al. Instability and excessive mechanical loading mediate subchondral bone changes to induce osteoarthritis. Ann Transl Med 8:350 (2020). PubMed: 32355794
- Zhou T et al. Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin. Elife 9:N/A (2020). PubMed: 32186512
- Lei L et al. Loss of receptor tyrosine kinase-like orphan receptor 2 impairs the osteogenesis of mBMSCs by inhibiting signal transducer and activator of transcription 3. Stem Cell Res Ther 11:137 (2020). PubMed: 32216811
- Hou Y et al. G-CSF partially mediates bone loss induced by Staphylococcus aureus infection in mice. Clin Sci (Lond) 133:1297-1308 (2019). PubMed: 31175224
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552) This image is courtesy of an anonymous collaborator
ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552) This image is courtesy of Jérôme AMIAUD, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives. We thank them for their important contribution to the validation of ab22552.
Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552) Image is courtesy of Dr Faye Doherty
ab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552) Image courtesy of Human Protein Atlas
Image courtesy of Human Protein Atlas
ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (ab22552) This image is courtesy of an anonymous AbreviewThe image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.
