Anti-MCM3 antibody - C-terminal (ab228778)
Key features and details
- Rabbit polyclonal to MCM3 - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MCM3 antibody - C-terminal
See all MCM3 primary antibodies -
Description
Rabbit polyclonal to MCM3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human MCM3 (C terminal). The exact sequence is proprietary.
Database link: P25205 -
Positive control
- WB: HEK-293T whole cell lysate. IHC: NCIC87 xenograft. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM3 antibody - C-terminal (ab228778)Anti-MCM3 antibody - C-terminal (ab228778) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 91 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-MCM3 antibody - C-terminal (ab228778)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for MCM3 (green) using ab228778 at 1/500 in ICC/IF.
Nuclear counterstain: Hoechst 33343 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody - C-terminal (ab228778)Paraffin-embedded NCIN87 xenograft stained for MCM3 using ab228778 at 1/100 dilution in immunohistochemical analysis.
