Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MCM3 antibody [EPR7080] (ab128923)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7080] to MCM3
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MCM3 antibody [EPR7080]
    See all MCM3 primary antibodies

  • Description

    Rabbit monoclonal [EPR7080] to MCM3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human MCM3 (P25205).

  • Positive control

    • L6, C6, RAW 264.7, PC-12, NIH/3T3, HeLa, K562, HepG2, U937 and 293T cell lysates. Human colon and tonsil tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7080

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MCM3 antibody [EPR7080] (ab128923)
    Western blot - Anti-MCM3 antibody [EPR7080] (ab128923)
    All lanes : Anti-MCM3 antibody [EPR7080] (ab128923) at 1/1000 dilution

    Lane 1 : L6 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : RAW 264.7 cell lysate
    Lane 4 : PC-12 cell lysate
    Lane 5 : NIH/3T3 cell lysate
    Lane 6 : HeLa cell lysate
    Lane 7 : K562 cell lysate
    Lane 8 : HepG2 cell lysate
    Lane 9 : U937 cell lysate
    Lane 10 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 91 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 at 1/100 dilution staining MCM3 in paraffin-embedded Human tonsil tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 showing positive staining in Breast carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 showing positive staining in Lung carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-MCM3 antibody [EPR7080] (ab128923)
    Flow Cytometry - Anti-MCM3 antibody [EPR7080] (ab128923)

    Flow Cytometry analysis of K562 (human chronic myelogenous leukemia) cells labeling MCM3 with unpurified ab128923 at 1/900 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM3 antibody [EPR7080] (ab128923)

    ab128923 showing positive staining in Cervical carcinoma tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-MCM3 antibody [EPR7080] (ab128923)
    Anti-MCM3 antibody [EPR7080] (ab128923)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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