Human PON1 ELISA Kit (ab277403)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PON1 ELISA Kit
See all PON1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 70.41 68% - 73% Serum 70.74 68% - 78% Plasma 72.72 67% - 90% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human PON1 ELISA Kit (ab277403) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PON1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human PON1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PON1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PON1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PON1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human PON1 Antibody 2 vials Anti-Human PON1 coated Microplate 1 unit Human PON1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. -
Tissue specificity
Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. -
Involvement in disease
Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5) [MIM:612633]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. -
Sequence similarities
Belongs to the paraoxonase family. -
Post-translational
modificationsGlycosylated.
The signal sequence is not cleaved.
Present in two forms, form B contains a disulfide bond, form A does not. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- A esterase 1
- A-esterase 1
- Aromatic esterase 1
see all -
Database links
- Entrez Gene: 5444 Human
- Omim: 168820 Human
- SwissProt: P27169 Human
- Unigene: 370995 Human
Images
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Human PON1 ELISA kit (ab277403) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.