Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210)
Suitable for: WB, IHC-P, Dot blot
Reacts with: Human

Product name

Anti-PLK1 (phospho T210) antibody [EPNCIR167]
See all PLK1 primary antibodies
Description

Rabbit monoclonal [EPNCIR167] to PLK1 (phospho T210)
Host species

Rabbit
Specificity

ab155095 only detects PLK1 phosphorylated at Threonine 210.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human PLK1.
Positive control
- HeLa lysate, treated with calyculin A; Human colon tissue and gastric carcinoma tissue.
General notes
This antibody was developed as part of a collaboration between the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPNCIR167
Isotype

IgG
Research areas

Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution

Lane 1 : Hela (Human cervix adenocarcinoma epithelial cell) whole cell lysates with NFDM/TBST
Lane 2 : Hela (Human cervix adenocarcinoma epithelial cell) treated with thymidine (2mM, 16 h) then with nocodazole (10nM, 24h). Whole cell lysates with NFDM/TBST
Lane 3 : Hela (Human cervix adenocarcinoma epithelial cell) treated with thymidine (2mM, 16 h) then with nocodazole (10nM, 24h). Whole cell lysates.Then the membrane was incubated with phosphatase. with NFDM/TBST

Lysates/proteins at 15 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 10 seconds

antibody used for ab155095 is purified batch
Dot Blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) Dot Blot. Primary ab dilution 1:1000, Secondary ab description and code (ab id)Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000. Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST. Lane 1:PLK1 (pT210) phospho peptide, Lane 2: PLK1 non-phospho peptide, Exposure time 10 seconds. Note: antibody used for ab155095 is purified batch.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

ab155095 staining PLK1 (phospho T210) in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/500). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.
Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate post treatment with Nocodazole

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

Predicted band size: 68 kDa
Western blot - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

All lanes : Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate, untreated
Lane 2 : HeLa cell lysate, treated with calyculin A

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Immunohistochemical analysis of paraffin-embedded Human gastroic carcinoma tissue labeling PLK1 with ab155095, unpurified, at 1/100 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab155095, unpurified, showing +ve staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155095, unpurified, showing +ve staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

Immunohistochemical analysis of paraffin embedded Human placenta tissue using ab155095, unpurified, showing +ve staining.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-PLK1 (phospho T210) antibody [EPNCIR167] (ab155095)

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