HRP Anti-PLK1 antibody [35-206] (ab203647)
![HRP Anti-PLK1 antibody [35-206] (ab203647)](https://www.abcam.com/ps/products/203/ab203647/Images/ab203647-265669-WBab2036471.jpg "HRP Anti-PLK1 antibody [35-206] (ab203647)")
Key features and details
- HRP Mouse monoclonal [35-206] to PLK1
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-PLK1 antibody [35-206]
See all PLK1 primary antibodies -
Description
HRP Mouse monoclonal [35-206] to PLK1 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human PLK1. His-PLK1 full length purified from Sf9 cells.
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Epitope
aa330-370. -
Positive control
- WB: A431 and HEK293 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
35-206 -
Isotype
IgG2b -
Research areas
Images
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Western blot - HRP Anti-PLK1 antibody [35-206] (ab203647)All lanes : HRP Anti-PLK1 antibody [35-206] (ab203647) at 1/1000 dilution
Lane 1 : A431 (Human) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203647 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
