Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [14/SATB1] to SATB1 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SATB1 antibody [14/SATB1] - BSA and Azide free
    See all SATB1 primary antibodies

  • Description

    Mouse monoclonal [14/SATB1] to SATB1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat thymus tissue lysate; Jurkat, THP1 cell lysates. IHC-P: Human thymus, tonsil; Mouse thymus; Rat thymus.

  • General notes

    ab280581 is the carrier-free version of ab280578. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280581 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Western blot - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    All lanes : Anti-SATB1 antibody [14/SATB1] (ab280578) at 1/1000 dilution

    Lane 1 : Rat thymus tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : THP1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 5 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 85 kDa



    This data was developed using ab280578, the same antibody clone in a different buffer formulation. 

    Blocking buffer and Diluting buffer concentration: 5% NFDM/TBST

    Negative control: liver tissue (PMID: 1505028).

    Exposure time: 15 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human thymus tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human thymus. The section was incubated with ab280578 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on human tonsil. The section was incubated with ab280578 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse thymus. The section was incubated with ab280578 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat thymus tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat thymus. The section was incubated with ab280578 for 30 mins at room temperature and followed by mouse specific IgG antibody (ab125913) for 8 mins.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the liver hepatocytes. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the liver hepatocytes. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

    This data was developed using ab280578 the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling SATB1 with ab280578 at 1/1000 (0.97 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on the liver hepatocytes. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)
    Anti-SATB1 antibody [14/SATB1] - BSA and Azide free (ab280581)

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