Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PLK1 antibody [35-206] - BSA and Azide free (ab264082)

Key features and details

  • Mouse monoclonal [35-206] to PLK1 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, ICC, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-PLK1 antibody [35-206] - BSA and Azide free
    See all PLK1 primary antibodies

  • Description

    Mouse monoclonal [35-206] to PLK1 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant full length protein corresponding to Human PLK1.

  • Epitope

    aa330-370.

  • Positive control

    • WB: A431, U-2 OS and HeLa S3 lysates. Flow cyt: HCT 116 cells. ICC/IF: U-2 OS, HeLa S3 and NIH/3T3 cells.

  • General notes

    ab264081 is a PBS only version of ab17056.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-PLK1 antibody [35-206] (ab264082)
    Flow Cytometry - Anti-PLK1 antibody [35-206] (ab264082)

    Overlay histogram showing HCT116 cells stained with ab17056 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17056, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different formulation containing PBS, Azide and Arginine (ab17056).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 antibody [35-206] - BSA and Azide free (ab264082)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 antibody [35-206] - BSA and Azide free (ab264082)

    Immunofluoresence using ab17056 and either U2OS, HeLaS3 or NIH 3T3 cells.

    Plk1 can be seen localising with MKLP in the U2OS cell and with Cdc20 in the HeLaS3 and NIH 3T3 cells shown.

    This data was developed using the same antibody clone in a different formulation containing PBS, Azide and Arginine (ab17056).

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