Anti-PLK1 antibody [36-298] - BSA and Azide free (ab178666)
![Anti-PLK1 antibody [36-298] - BSA and Azide free (ab178666)](https://www.abcam.com/ps/products/178/ab178666/Images/ab178666-284259-anti-plk1-antibody-36-298-flow-cytometry.jpg "Anti-PLK1 antibody [36-298] - BSA and Azide free (ab178666)")
Key features and details
- Mouse monoclonal [36-298] to PLK1 - BSA and Azide free
- Suitable for: WB, IP, Flow Cyt, Indirect ELISA
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-PLK1 antibody [36-298] - BSA and Azide free
See all PLK1 primary antibodies -
Description
Mouse monoclonal [36-298] to PLK1 - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant full length protein corresponding to Human PLK1.
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Epitope
aa330-370. -
Positive control
- WB: 293, HeLaS3 or U2OS cell lysate ICC: HeLaS3, NIH 3T3 or U2OS cells
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
36-298 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-PLK1 antibody [36-298] - BSA and Azide free (ab178666)
This flow cytometry data was generated using the same antibody clone in a different buffer formulation (ab17057).
Overlay histogram showing U20S cells stained with ab17057 (red line). The cells were fixed with 80% methanol (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab17057, 1 µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was mouse IgG1 [15-6E10A7] (ab170190, 1 µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
