Anti-PLK1 (phospho S137) antibody (ab21738)
Key features and details
- Rabbit polyclonal to PLK1 (phospho S137)
- Suitable for: ICC/IF, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLK1 (phospho S137) antibody
See all PLK1 primary antibodies -
Description
Rabbit polyclonal to PLK1 (phospho S137) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human PLK1 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab25712) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab21738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 5 µg/ml. AP-791911
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ELISA Use at an assay dependent concentration. Target
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Function
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. -
Tissue specificity
Placenta and colon. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain. -
Developmental stage
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. -
Post-translational
modificationsCatalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. During early stages of mitosis, the phosphorylated form is detected on centrosomes and kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the phosphorylated form of BUB1 is required for the kinetochore localization. - Information by UniProt
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Database links
- Entrez Gene: 5347 Human
- Entrez Gene: 18817 Mouse
- Entrez Gene: 25515 Rat
- Entrez Gene: 380481 Xenopus laevis
- Omim: 602098 Human
- SwissProt: P53350 Human
- SwissProt: Q07832 Mouse
- SwissProt: Q62673 Rat
see all -
Alternative names
- Cell cycle regulated protein kinase antibody
- PLK 1 antibody
- PLK antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PLK1 (phospho S137) antibody (ab21738)
ICC/IF image of ab21738 stained MCF7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21738, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho S137) antibody (ab21738)IHC image of PLK1 (phospho S137) staining in human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21738, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX -
ELISA - Anti-PLK1 (phospho S137) antibody (ab21738)
Protocols
References (4)
ab21738 has been referenced in 4 publications.
- Shin SB et al. Active PLK1-driven metastasis is amplified by TGF-ß signaling that forms a positive feedback loop in non-small cell lung cancer. Oncogene 39:767-785 (2020). PubMed: 31548612
- Watts E et al. Designing Dual Inhibitors of Anaplastic Lymphoma Kinase (ALK) and Bromodomain-4 (BRD4) by Tuning Kinase Selectivity. J Med Chem 62:2618-2637 (2019). PubMed: 30789735
- Martínez-León E et al. Protein kinase D1 inhibition interferes with mitosis progression. J Cell Physiol N/A:N/A (2019). PubMed: 30997696
- Diab A et al. Polo-like-kinase 1 is a proviral host factor for hepatitis B virus replication. Hepatology 66:1750-1765 (2017). PubMed: 28445592
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PLK1 (phospho S137) antibody (ab21738)
ICC/IF image of ab21738 stained MCF7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21738, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLK1 (phospho S137) antibody (ab21738)IHC image of PLK1 (phospho S137) staining in human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21738, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
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ELISA - Anti-PLK1 (phospho S137) antibody (ab21738)
