Anti-LINGO3 antibody [EPR9635] (ab169772)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9635] to LINGO3
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-LINGO3 antibody [EPR9635]
See all LINGO3 primary antibodies -
Description
Rabbit monoclonal [EPR9635] to LINGO3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide corresponding to residues in Human LINGO3 (UniProt P0C6S8).
-
Positive control
- SH-SY5Y, U87-MG, 293T and NCI-H460 cell lysates; 293T cells; SH-SY5Y cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9635 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-LINGO3 antibody [EPR9635] (ab169772) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : 293T cell lysate
Lane 4 : NCI-H460 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 65 kDa
-
Immunofluorescent analysis of 293T cells labeling LINGO3 with ab169772 at 1/250 dilution (green). DAPI nuclear staining (blue).
-
Flow cytometry analysis of permeabilized SH-SY5Y cells labeling LINGO3 with ab169772 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
-
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab169772.
-