Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13603] to SAMD9 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SAMD9 antibody [EPR13603] - BSA and Azide free
    See all SAMD9 primary antibodies

  • Description

    Rabbit monoclonal [EPR13603] to SAMD9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, A431 and MCF7 cell lysates. Flow Cyt: A431 and MCF7 cells. ICC: A431 cells.

  • General notes

    Ab250226 is the carrier-free version of ab180575. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250226 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13603

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Western blot - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    All lanes : Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : SAMD9 knockout A549 cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 184 kDa
    Observed band size: 184 kDa



    This data was developed using ab180575, the same antibody clone in a different buffer formulation.

    Lanes 1-3: Merged signal (red and green). Green - ab180575 observed at 184 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab180575 Anti-SAMD9 antibody [EPR13603]  was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267039 (knockout cell lysate ab257657) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Immunocytochemistry/ Immunofluorescence - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1:50 dilution (5.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with none. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti samd9 antibody epr13603 immunocytochemistry a431 human)
  • Western blot - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Western blot - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    All lanes : Anti-SAMD9 antibody [EPR13603] (ab180575) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : SAMD9 knockout A549 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human skeletal muscle cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 184 kDa
    Observed band size: 184 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab180575).

    Lanes 1-4: Merged signal (red and green). Green - ab180575 observed at 184 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab180575 Anti-SAMD9 antibody [EPR13603] was shown to specifically react with SAMD9 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267038 (knockout cell lysate ab257656) was used. Wild-type and SAMD9 knockout samples were subjected to SDS-PAGE. ab180575 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Flow Cytometry - Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Flow Cytometry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling SAMD9 with purified ab180575 at 1/30 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180575)
  • Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)
    Anti-SAMD9 antibody [EPR13603] - BSA and Azide free (ab250226)

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