Anti-C10orf35 antibody [EP14950-82] (ab189923)
![Anti-C10orf35 antibody [EP14950-82] (ab189923)](https://www.abcam.com/ps/products/189/ab189923/Images/ab189923-225665-anti-c10orf35-antibody-ep14950-82-western-blot.jpg "Anti-C10orf35 antibody [EP14950-82] (ab189923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP14950-82] to C10orf35
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-C10orf35 antibody [EP14950-82]
See all C10orf35 primary antibodies -
Description
Rabbit monoclonal [EP14950-82] to C10orf35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human C10orf35 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q96D05 -
Positive control
- Human fetal brain, fetal kidney, MCF7, A431, Mouse and rat brain lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP14950-82 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-C10orf35 antibody [EP14950-82] (ab189923)All lanes : Anti-C10orf35 antibody [EP14950-82] (ab189923) at 1/50000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : MCF7 lysate
Lane 4 : A431 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
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![Western blot - Anti-C10orf35 antibody [EP14950-82] (ab189923)](https://www.abcam.com/ps/products/189/ab189923/Images/ab189923-225667-anti-c10orf35-antibody-ep14950-82-western-blot.jpg)
Western blot - Anti-C10orf35 antibody [EP14950-82] (ab189923)All lanes : Anti-C10orf35 antibody [EP14950-82] (ab189923) at 1/10000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 13 kDa
Observed band size: 13 kDa
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![Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] (ab189923)](https://www.abcam.com/ps/products/189/ab189923/Images/ab189923-225669-anti-c10orf35-antibody-ep14950-82-immunoprecipitation.jpg)
Immunoprecipitation - Anti-C10orf35 antibody [EP14950-82] (ab189923)Western blot analysis of C10orf35 in Human fetal brain lysate immunoprecipitated using ab189923 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.