Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9198(2)] to SNAP29 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free
    See all SNAP29 primary antibodies

  • Description

    Rabbit monoclonal [EPR9198(2)] to SNAP29 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and HeLa cell lysates. IP: Jurkat cell lysate. ICC/IF: HeLa cells. Flow Cyt: Jurkat cell lysate.

  • General notes

    Ab240248 is the carrier-free version of ab181151. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240248 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SNAP29 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab181151).

    Lanes 1-3: Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used.  Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE.  ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)
    Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)

    Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181151).

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)

    Immunofluorescent analysis of -20? acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181151).

  • Flow Cytometry - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)
    Flow Cytometry - Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)

    Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181151).

  • Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)
    Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free (ab240248)

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