Anti-SNAP29 antibody - C-terminal (ab229584)
Key features and details
- Rabbit polyclonal to SNAP29 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SNAP29 antibody - C-terminal
See all SNAP29 primary antibodies -
Description
Rabbit polyclonal to SNAP29 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant fragment within Human SNAP29 (C terminal). The exact sequence is proprietary.
Database link: O95721 -
Positive control
- WB: Mouse and rat brain tissue extracts; HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAP29 antibody - C-terminal (ab229584)All lanes : Anti-SNAP29 antibody - C-terminal (ab229584) at 1/500 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Predicted band size: 29 kDa12% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody - C-terminal (ab229584)4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for SNAP29 (green) using ab229584 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with DAPI (blue). beta Tubulin 3/ Tuj1, a neuron cell marker, stained by anti-beta Tubulin 3/ Tuj1 antibody at 1/500 dilution.
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Western blot - Anti-SNAP29 antibody - C-terminal (ab229584)All lanes : Anti-SNAP29 antibody - C-terminal (ab229584) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa12% SDS-PAGE gel.
