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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs

Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

Price and availability

268 032 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E377] to Synapsin I (phospho S553)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Synapsin I (phospho S553) antibody [E377]
    See all Synapsin I primary antibodies
  • Description

    Rabbit monoclonal [E377] to Synapsin I (phospho S553)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Synapsin phosphorylated on Serine 553
  • Tested Applications & Species

    Application Species
    Dot
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding Ser553 of Synapsin (Human)

  • Positive control

    • WB: Untreated Human fetal brain, Mouse brain lysates, and Human fetal brain treated with alkaline phosphatase lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E377
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

Images

  • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution (Purified)

    Lane 1 : Untreated Human fetal brain lysates
    Lane 2 : Human fetal brain lysates treated with alkaline phosphatase

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

    Lane 1 : Untreated rat brain whole cell lysates
    Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking buffer: 2% BSA/TBST

    Dilution buffer: 2% BSA/TBST

  • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution

    Lane 1 : Untreated mouse brain whole cell lysate
    Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    Western blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution

    Lane 1 : Untreated mouse brain whole cell lysate
    Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 74 kDa
    Observed band size: 77 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking buffer: 2% BSA/TBST

    Dilution buffer: 2% BSA/TBST

  • Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    Dot Blot - Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

    Dot Blot analysis on human immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500

  • Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
    Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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