Anti-Synapsin I (phospho S553) antibody [E377] (ab32532)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E377] to Synapsin I (phospho S553)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Synapsin I (phospho S553) antibody [E377]
See all Synapsin I primary antibodies -
Description
Rabbit monoclonal [E377] to Synapsin I (phospho S553) -
Host species
Rabbit -
Specificity
This antibody only detects Synapsin phosphorylated on Serine 553 -
Tested Applications & Species
See all applications and species dataApplication Species Dot HumanWB MouseRatHuman -
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser553 of Synapsin (Human)
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Positive control
- WB: Untreated Human fetal brain, Mouse brain lysates, and Human fetal brain treated with alkaline phosphatase lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E377 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution (Purified)
Lane 1 : Untreated Human fetal brain lysates
Lane 2 : Human fetal brain lysates treated with alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
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All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution
Lane 1 : Untreated rat brain whole cell lysates
Lane 2 : Rat brain whole cell lysates, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
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All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/1000 dilution
Lane 1 : Untreated mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysate, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-Synapsin I (phospho S553) antibody [E377] (ab32532) at 1/200 dilution
Lane 1 : Untreated mouse brain whole cell lysate
Lane 2 : Mouse brain whole cell lysates, treated with alkaline phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking buffer: 2% BSA/TBST
Dilution buffer: 2% BSA/TBST
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Dot Blot analysis on human immunogen phospho-peptide (A) and non-phospho peptide (B) using ab32532 at a dilution of 1/500
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