Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Synapsin I antibody - Synaptic Marker (ab64581)

Key features and details

  • Rabbit polyclonal to Synapsin I - Synaptic Marker
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Goat
  • Isotype: IgG

Overview

  • Product name

    Anti-Synapsin I antibody - Synaptic Marker
    See all Synapsin I primary antibodies

  • Description

    Rabbit polyclonal to Synapsin I - Synaptic Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat Synapsin I aa 600-700 conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    (Peptide available as ab64580)

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Mouse
WB
Mouse
Rat
All applications
Sheep
Human
Application Abreviews Notes
WB (3)
Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa).

Abcam recommends using 3-5% milk as the blocking agent.
IP
Use at an assay dependent concentration.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 74, 70 kDa (predicted molecular weight: 74 kDa).

Abcam recommends using 3-5% milk as the blocking agent.
IP
Use at an assay dependent concentration.

Target

  • Function

    Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.

  • Involvement in disease

    Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders [MIM:300491]. XELBD is characterized by variable combinations of epilepsy, learning difficulties, macrocephaly, and aggressive behavior.

  • Sequence similarities

    Belongs to the synapsin family.

  • Post-translational
    modifications

    Substrate of at least four different protein kinases. It is probable that phosphorylation plays a role in the regulation of synapsin-1 in the nerve terminal. Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Cell junction > synapse. Golgi apparatus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Brain protein 4.1 antibody
    • SYN 1 antibody
    • SYN 1a antibody
    • SYN 1b antibody
    • SYN I antibody
    • SYN1 antibody
    • SYN1_HUMAN antibody
    • SYN1a antibody
    • SYN1b antibody
    • Synapsin 1 antibody
    • Synapsin I antibody
    • Synapsin-1 antibody
    • Synapsin1 antibody
    • SynapsinI antibody
    • SYNI antibody
    see all

Images

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)This image is courtesy of an anonymous Abreview
    Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg

    Secondary
    Donkey anti-Rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 80,85 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocked with 5% milk for 1 hour at 25°C.

    Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.

    See Abreview

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 70,74 kDa why is the actual band size different from the predicted?



    The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data).
  • Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.

References (48)

Publishing research using ab64581? Please let us know so that we can cite the reference in this datasheet.

ab64581 has been referenced in 48 publications.

  • Sharma Y  et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
  • Cha M  et al. Astroglial changes in the zona incerta in response to motor cortex stimulation in a rat model of chronic neuropathy. Sci Rep 10:943 (2020). PubMed: 31969638
  • Mandai T  et al. T-495, a novel low cooperative M1 receptor positive allosteric modulator, improves memory deficits associated with cholinergic dysfunction and is characterized by low gastrointestinal side effect risk. Pharmacol Res Perspect 8:e00560 (2020). PubMed: 31990455
  • Pagella P  et al. Ameloblastomas Exhibit Stem Cell Potential, Possess Neurotrophic Properties, and Establish Connections with Trigeminal Neurons. Cells 9:N/A (2020). PubMed: 32155948
  • Wu Q  et al. SRC-1 Knockout Exerts No Effect on Amyloid ß Deposition in APP/PS1 Mice. Front Aging Neurosci 12:145 (2020). PubMed: 32625077
View all Publications for this product

Images

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581) This image is courtesy of an anonymous Abreview
    Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1/5000 dilution + Rat whole brain tissue lysate at 15 µg

    Secondary
    Donkey anti-Rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 80,85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocked with 5% milk for 1 hour at 25°C.

    Sample was incubated with primary antibody for 18 hours at 4°C in TBS + 0.1% Tween.

    See Abreview

  • Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Western blot - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    All lanes : Anti-Synapsin I antibody - Synaptic Marker (ab64581) at 1 µg/ml

    Lane 1 : Spinal Cord (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate - normal tissue

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 74 kDa
    Observed band size: 70,74 kDa why is the actual band size different from the predicted?



    The immunogen for ab64581 is predicted to recognise both the 70 kDa and 74 kDa isoforms of Synapsin I. Synapsin I is also known to contain a number of potential phosphorylation and glycosylation sites (SwissProt data).
  • Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Immunoprecipitation - Anti-Synapsin I antibody - Synaptic Marker (ab64581)
    Synapsin I - Synaptic Marker was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Synapsin I - Synaptic Marker and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab64581.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 80kDa: Synapsin I - Synaptic Marker contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band.

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