Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human placenta tissue lysate; His-tagged human Galectin 13/PLAC8 recombinant protein; His-lif tagged human Galectin 16 recombinant protein (aa 1-141). IHC-P: Human placenta tissue. IP: Human placenta tissue lysate.

  • General notes

    ab273575 is the carrier-free version of ab272716. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Galectin 13+Galectin 16 with ab272716 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 25191322). The section was incubated with ab272716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272716).

  • Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)
    Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)

    Galectin 13+Galectin 16 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab272716 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

    Lane 1: Human placenta tissue lysate 10µg

    Lane 2: ab272716 IP in human placenta tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272716 in human placenta tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272716).

  • Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)
    Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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