Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)
![Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)](https://www.abcam.com/ps/products/273/ab273575/Images/ab273575-1-anti-galectin-13galectin-16-antibody-epr2338389-immunohistochemistry-human-placenta.jpg "Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16 - BSA and Azide free
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free -
Description
Rabbit monoclonal [EPR23383-89] to Galectin 13/PLAC8 + Galectin-16 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human placenta tissue lysate; His-tagged human Galectin 13/PLAC8 recombinant protein; His-lif tagged human Galectin 16 recombinant protein (aa 1-141). IHC-P: Human placenta tissue. IP: Human placenta tissue lysate.
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General notes
ab273575 is the carrier-free version of ab272716. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23383-89 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Galectin 13+Galectin 16 with ab272716 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placenta (PMID: 25191322). The section was incubated with ab272716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272716).
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![Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)](https://www.abcam.com/ps/products/273/ab273575/Images/ab273575-2-anti-galectin-13galectin-16-antibody-epr2338389-immunoprecipitation-human-placenta-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)Galectin 13+Galectin 16 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab272716 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.
Lane 1: Human placenta tissue lysate 10µg
Lane 2: ab272716 IP in human placenta tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272716 in human placenta tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab272716).
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![Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)](https://www.abcam.com/ps/products/273/ab273575/Images/ab273575-3-benefits-of-recombinant-antibodies.png)
Anti-Galectin 13/PLAC8 + Galectin-16 antibody [EPR23383-89] - BSA and Azide free (ab273575)