Human Ficolin-2 ELISA Kit (ab275554)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.35 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Ficolin-2 ELISA Kit
See all Ficolin 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 0.1% Inter-assay Sample n Mean SD CV% Serum 3 7% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.35 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 91 86% - 100% Cell culture media 102 98% - 107% Hep Plasma 110 101% - 115% EDTA Plasma 105 104% - 107% Cit plasma 85 82% - 89% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Ficolin-2 ELISA kit (ab275554) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Ficolin-2 protein in serum, plasma and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ficolin-2 with 8.35 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Ficolin-2, initially identified as L-ficolin, in human is encoded by the FCN2 gene. Ficolin-2 belongs to the ficolin family of proteins and is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolin-2 is predominantly expressed in the liver. Studies have reported it to have roles in carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in the ficolin-2 serum concentration.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Ficolin-2 Capture Antibody 1 x 600µl 10X Human Ficolin-2 Detector Antibody 1 x 600ml 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Ficolin-2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc-binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the ficolin lectin family.
Contains 1 collagen-like domain.
Contains 1 fibrinogen C-terminal domain. -
Domain
The fibrinogen-like domain (FBG) contains potential calcium-binding sites that may be involved in carbohydrate binding. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 37 kDa elastin-binding protein
- Collagen/fibrinogen domain containing protein 2
- Collagen/fibrinogen domain-containing protein 2
see all -
Database links
- Entrez Gene: 2220 Human
- Entrez Gene: 2220 Human
- Omim: 601624 Human
- SwissProt: Q15485 Human
- SwissProt: Q15485 Human
- Unigene: 54517 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Ficolin-2 standard curve in Sample Diluent NS.The Ficolin-2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Ficolin-2 in human serum and plasma samples.The concentrations of Ficolin-2 were measured in duplicates, interpolated from the Ficolin-2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:200, plasma (citrate) 1:200, plasma (EDTA) 1:400, and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ficolin-2 concentration was determined to be 481.67 ng/mL in serum, 508.04 ng/mL in plasma (citrate), 443.89 ng/mL in plasma (EDTA), and 500.03 ng/mL in plasma (heparin).
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Serum diluted to 1:400 from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Ficolin-2 concentration was determined to be 403.80 ng/mL with a range of 138.63 – 755.53 ng/mL.
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Sandwich ELISA - Human Ficolin-2 ELISA Kit (ab275554)To learn more about the advantages of recombinant antibodies see here.
