Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23146-225] to Ficolin 2
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Ficolin 2 antibody [EPR23146-225]
    See all Ficolin 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23146-225] to Ficolin 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma; human liver lysate; Human Ficolin-2 recombinant protein (aa26- 313). IP: Human plasma.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23146-225

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)
    Western blot - Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)
    All lanes : Anti-Ficolin 2 antibody [EPR23146-225] (ab267473) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Human liver tissue lysate
    Lane 3 : Human brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 34 kDa



    FCN2 has a high molecular weight (400 kDa) due to extensive disulfide bridge formation (PMID: 16595153)

    Negative control: Human brain (PMID: 8576206)

    A weak unidentifiable band was observed in brain tissue

    Blocking/diluting buffer and concentration: 5% NFDM/TBST

  • Immunoprecipitation - Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)
    Immunoprecipitation - Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)

    Ficolin 2 was immunoprecipitated from 0.35 mg Human plasma with ab267473 at 1/30 dilution . Western blot was performed on the immunoprecipitate using ab267473 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP(ab131366) was used at 1/5000 dilution.

    Lane 1: Human plasma lysate 10 μg. 
    Lane 2: ab267473 IP in Human plasma.  
    Lane 3: Rabbit monoclonal IgG isotype control (ab172730) instead of ab267473 in Human plasma.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7.75 seconds

    Ficolin 2 has a high molecular weight (250 kDa) due to extensive disulfide bridge formation (PMID: 16595153).

  • Western blot - Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)
    Western blot - Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)
    All lanes : Anti-Ficolin 2 antibody [EPR23146-225] (ab267473) at 1/1000 dilution

    Lane 1 : Human Ficolin 2 recombinant Protein (aa26- 313) 20 ng
    Lane 2 : Human Ficolin 1 recombinant Protein (aa28- 326) 20 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 34 kDa



    Blocking/diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 5.5 seconds

  • Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)
    Anti-Ficolin 2 antibody [EPR23146-225] (ab267473)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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