Anti-Synapsin I antibody (ab18814)
Key features and details
- Rabbit polyclonal to Synapsin I
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Synapsin I antibody
See all Synapsin I primary antibodies -
Description
Rabbit polyclonal to Synapsin I -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Synapsin I. Corresponds to residues surrounding amino acid 12 of Synapsin I.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Mouse brain whole cell lysate - untreated
Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
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Western blot - Anti-Synapsin I antibody (ab18814)All lanes : Anti-Synapsin I antibody (ab18814) at 1/5000 dilution
Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Controls - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) and Anti-GAPDH antibody [EPR16891] (ab181602).
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Western blot - Anti-Synapsin I antibody (ab18814)Anti-Synapsin I antibody (ab18814) at 1 µg/ml + Mouse brain tissue lysate.
Predicted band size: 74 kDa
Observed band size: ~77 kDa why is the actual band size different from the predicted?Secondary antibody - goat anti-rabbit HRP (ab6721)
