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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsc70 antibody (ab223611)

Anti-Hsc70 antibody (ab223611)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsc70
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsc70 antibody
    See all Hsc70 primary antibodies
  • Description

    Rabbit polyclonal to Hsc70
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hsc70 aa 550-646 (C terminal).
    Database link: P11142

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab223611)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab223611)

    Mouse backskin tissue stained for Hsc70 using ab223611 at 1/100 dilution for 1 hour at room temperature in immunohistochemical analysis, followed by Goat Anti-Rabbit FITC (green) at 1/50 for 1 hour at room temperature. Fixation: Bouin’s Fixative Solution.

  • Western blot - Anti-Hsc70 antibody (ab223611)
    Western blot - Anti-Hsc70 antibody (ab223611)
    Anti-Hsc70 antibody (ab223611) at 1/1000 dilution + heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysates at 15 µg

    Secondary
    Donkey Anti-Rabbit IgG HRP

    Predicted band size: 70 kDa



    Blocking buffer: 1.5% BSA for 30 minutes at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab223611)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab223611)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsc70 using ab223611 at 1/10000 dilution for 12 hours at 4°C in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 for 1 hour at room temperature. Counterstain: Methyl Green for 2 minutes at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab223611)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody (ab223611)

    Formalin-fixed, paraffin-embedded mouse inflamed colon tissue stained for Hsc70 using ab223611 at 1/50000 dilution for 12 hours at 4°C in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 for 1 hour at room temperature. Counterstain: Methyl Green for 2 minutes at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Hsc70 antibody (ab223611)

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    Anti-Hsc70 antibody [EP1531Y] - BSA and Azide free (ab220821)

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    Anti-Hsc70 antibody (ab112549)

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    Anti-Hsc70 antibody [EP1531Y] (ab51052)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    HRP Anti-Hsc70 antibody [EP1531Y] (ab200757)

    Applications: WB

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    Anti-Hsc70 antibody (ab125307)

    Applications: ICC/IF, IHC-P, WB

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