Anti-Hsc70 antibody (ab223611)
Key features and details
- Rabbit polyclonal to Hsc70
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsc70 antibody
See all Hsc70 primary antibodies -
Description
Rabbit polyclonal to Hsc70 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Hsc70 aa 550-646 (C terminal).
Database link: P11142 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Mouse backskin tissue stained for Hsc70 using ab223611 at 1/100 dilution for 1 hour at room temperature in immunohistochemical analysis, followed by Goat Anti-Rabbit FITC (green) at 1/50 for 1 hour at room temperature. Fixation: Bouin’s Fixative Solution.
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Anti-Hsc70 antibody (ab223611) at 1/1000 dilution + heat shocked HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysates at 15 µg
Secondary
Donkey Anti-Rabbit IgG HRP
Predicted band size: 70 kDaBlocking buffer: 1.5% BSA for 30 minutes at room temperature.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Hsc70 using ab223611 at 1/10000 dilution for 12 hours at 4°C in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 for 1 hour at room temperature. Counterstain: Methyl Green for 2 minutes at room temperature.
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Formalin-fixed, paraffin-embedded mouse inflamed colon tissue stained for Hsc70 using ab223611 at 1/50000 dilution for 12 hours at 4°C in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 for 1 hour at room temperature. Counterstain: Methyl Green for 2 minutes at room temperature.