Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15473] to Syntaxin 4
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Syntaxin 4 antibody [EPR15473]
    See all Syntaxin 4 primary antibodies

  • Description

    Rabbit monoclonal [EPR15473] to Syntaxin 4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, K562, HeLa and A431 cell lysates. Wild-type HAP1 cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Syntaxin 4 knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab184545 observed at 1X kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab184545 was shown to recognize Syntaxin 4 when Syntaxin 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Syntaxin 4 knockout samples were subjected to SDS-PAGE. ab184545 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    All lanes : Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/2000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Western blot - Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Anti-Syntaxin 4 antibody [EPR15473] (ab184545) at 1/10000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Anti-Syntaxin 4 antibody [EPR15473] (ab184545)
    Anti-Syntaxin 4 antibody [EPR15473] (ab184545)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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