Anti-Syntaxin 4 antibody - N-terminal (ab226218)
Key features and details
- Rabbit polyclonal to Syntaxin 4 - N-terminal
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin 4 antibody - N-terminal
See all Syntaxin 4 primary antibodies -
Description
Rabbit polyclonal to Syntaxin 4 - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human Syntaxin 4 aa 1-50 (N terminal). The exact sequence is proprietary. (NP_004595.2).
Database link: Q12846 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
ab226218 has not performed satisfactorily when used for WB of Syntaxin 4 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of Syntaxin 4.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226218 was affinity purified using an epitope specific to Syntaxin 4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Syntaxin 4 antibody - N-terminal (ab226218)
Syntaxin 4 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226218 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-Syntaxin 4 antibody at 1 µg/ml.
Lane 1: ab226218 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
