Anti-Synaptophysin antibody (ab32594)
Key features and details
- Rabbit polyclonal to Synaptophysin
- Suitable for: WB, ICC/IF
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Synaptophysin antibody
See all Synaptophysin primary antibodies -
Description
Rabbit polyclonal to Synaptophysin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat -
Immunogen
Synthetic peptide:
GPGGYGPQDSYGPQGGYQPD
, corresponding to amino acids 253-272 of Rat Synaptophysin -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Anti-Synaptophysin antibody (ab32594) at 1/1000 dilution + Apteronotus leptorhynchus brain tissue lysate at 50 µg
Secondary
AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG
at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 33,35 kDa why is the actual band size different from the predicted?
Exposure time: 7 minutes
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ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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ab32594 labelling Synaptophysin by Immunocytochemistry/Immunofluorescence.
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Anti-Synaptophysin antibody (ab32594) at 1/5000 dilution + Rat brain
Predicted band size: 34 kDa
Observed band size: 33.8 kDa why is the actual band size different from the predicted?