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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs

Anti-Use1 antibody (ab234867)

Anti-Use1 antibody (ab234867)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Use1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Use1 antibody
    See all Use1 primary antibodies
  • Description

    Rabbit polyclonal to Use1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Use1 aa 1-146.
    Database link: Q9NZ43

  • Positive control

    • WB: Mouse heart, liver, brain, stomach and skeletal muscle lysates; Jurkat whole cell lysate. IP: PC-3 whole cell lysate. ICC/IF: U-251 MG cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs

Images

  • Western blot - Anti-Use1 antibody (ab234867)
    Western blot - Anti-Use1 antibody (ab234867)
    All lanes : Anti-Use1 antibody (ab234867) at 1/500 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse liver lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Mouse stomach lysate
    Lane 6 : Mouse skeletal muscle lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 29 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Use1 antibody (ab234867)
    Immunocytochemistry/ Immunofluorescence - Anti-Use1 antibody (ab234867)

    U-251 MG (human brain glioma cell line) cells stained for Use1 (green) using ab234867 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitation - Anti-Use1 antibody (ab234867)
    Immunoprecipitation - Anti-Use1 antibody (ab234867)

    Use1 was immunoprecipitated from 0.5 mg PC-3 (human prostate adenocarcinoma cell line) whole cell lysate using ab234867 at 1/200 dilution. 

    Lane 1: Control IgG IP in PC-3 whole cell lysate.

    Lane 2: ab234867 IP in PC-3 whole cell lysate.

    Lane 3: PC-3 whole cell lysate 10 μg (Input).

    For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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