Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free (ab242403)
![Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free (ab242403)](https://www.abcam.com/ps/products/242/ab242403/Images/ab242403-332504-242403333.jpg "Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free (ab242403)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15473] to Syntaxin 4 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free
See all Syntaxin 4 primary antibodies -
Description
Rabbit monoclonal [EPR15473] to Syntaxin 4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, K562, HeLa and A431 cell lysates. Wild-type HAP1 cell lysate.
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General notes
Ab242403 is the carrier-free version of ab184545. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab242403 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15473 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free (ab242403)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Syntaxin 4 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab184545 observed at 1X kDa. Red - loading control, ab18058, observed at 124 kDa.
ab184545 was shown to recognize Syntaxin 4 when Syntaxin 4 knockout samples were used, along with additional cross-reactive bands. Wild-type and Syntaxin 4 knockout samples were subjected to SDS-PAGE. ab184545 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab184545).
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![Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free (ab242403)](https://www.abcam.com/ps/products/242/ab242403/Images/ab242403-2-benefits-of-recombinant-antibodies.png)
Anti-Syntaxin 4 antibody [EPR15473] - BSA and Azide free (ab242403)
