Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)
![Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)](https://www.abcam.com/ps/products/240/ab240110/Images/ab240110-393671-anti-snap29-antibody-epr9199-western-blot-human-knockout-lysate.jpg "Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9199] to SNAP29 - BSA and Azide free
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SNAP29 antibody [EPR9199] - BSA and Azide free
See all SNAP29 primary antibodies -
Description
Rabbit monoclonal [EPR9199] to SNAP29 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and HepG2 cell lysates. IP: HeLa cell lysate.
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General notes
Ab240110 is the carrier-free version of ab138500. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240110 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.30 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9199 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAP29 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab138500).
Lanes 1-3: Merged signal (red and green). Green - ab138500 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.
ab138500 Anti-SNAP29 antibody [EPR9199] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab138500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)](https://www.abcam.com/ps/products/240/ab240110/Images/ab240110-2-ab1385004ab138500IP1.jpg)
Immunoprecipitation - Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)ab138500 (purified) at 1/20 immunoprecipitating SNAP29 in 10 µg HeLa cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138500).
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![OI-RD Scanning - Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)](https://www.abcam.com/ps/products/240/ab240110/Images/ab240110-1-SNAP29Primaryantibodiesab1385003.jpg)
OI-RD Scanning - Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KDThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138500).
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![Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)](https://www.abcam.com/ps/products/240/ab240110/Images/ab240110-4-benefits-of-recombinant-antibodies.png)
Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)
