Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9198(2)] to SNAP29
Suitable for: WB, ICC/IF, Flow Cyt, IP
Knockout validated
Reacts with: Human

Product name

Anti-SNAP29 antibody [EPR9198(2)]
See all SNAP29 primary antibodies
Description

Rabbit monoclonal [EPR9198(2)] to SNAP29
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HepG2, 293 and Jurkat whole cell lysates. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9198(2)
Isotype

IgG
Research areas

Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAP29 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Lanes 1-3: Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Immunofluorescent analysis of -20℃ acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).
Flow Cytometry - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).
Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilution

Lane 1 : Hela cell lysate with NFDM/TBST
Lane 2 : 293 cell lysate with NFDM/TBST
Lane 3 : Jurkat cell lysate with NFDM/TBST

Lysates/proteins at 20 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa
Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST

Lane 1 : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/50 dilution

Lane 1 : Precipitate from Jurkat lysate using ab181151 with NFDM/TBST
Lane 2 : Jurkat lysate with NFDM/TBST

Blocking peptides at 5 % per lane.

Secondary
All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com