Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9198(2)] to SNAP29
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SNAP29 antibody [EPR9198(2)]
    See all SNAP29 primary antibodies

  • Description

    Rabbit monoclonal [EPR9198(2)] to SNAP29

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HepG2, 293 and Jurkat whole cell lysates. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SNAP29 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used.  Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE.  ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Immunofluorescent analysis of -20℃ acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor®555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).

  • Flow Cytometry - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Flow Cytometry - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilution

    Lane 1 : Hela cell lysate with NFDM/TBST
    Lane 2 : 293 cell lysate with NFDM/TBST
    Lane 3 : Jurkat cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST



    Lane 1 : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/50 dilution


    Lane 1 : Precipitate from Jurkat lysate using ab181151 with NFDM/TBST
    Lane 2 : Jurkat lysate with NFDM/TBST

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
  • Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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