Anti-Pallidin antibody (ab230361)
Key features and details
- Rabbit polyclonal to Pallidin
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Pallidin antibody
See all Pallidin primary antibodies -
Description
Rabbit polyclonal to Pallidin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment corresponding to Human Pallidin aa 1 to the C-terminus.
Database link: Q9UL45 -
Positive control
- WB: A431 and HEK-293T whole cell lysates. IHC-P: Human pancreatic cancer tissue. IP: HEK-293 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Research areas
Images
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Western blot - Anti-Pallidin antibody (ab230361)All lanes : Anti-Pallidin antibody (ab230361) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 20, 9 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pallidin antibody (ab230361)Paraffin-embedded human pancreatic cancer tissue stained for Pallidin using ab230361 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-Pallidin antibody (ab230361)Pallidin was immunoprecipitated from 500 µg HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate with 4 µg ab230361.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in HEK-293 whole cell lysate.
Lane 2: ab230361 IP in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody at 1/50000 dilution.
