Anti-SCML2 antibody [SCMAD14A] (ab51506)
![Anti-SCML2 antibody [SCMAD14A] (ab51506)](https://www.abcam.com/ps/products/51/ab51506/Images/ab51506-63460-anti-scml2-antibody-scmad14a-western-blot.jpg "Anti-SCML2 antibody [SCMAD14A] (ab51506)")
Key features and details
- Mouse monoclonal [SCMAD14A] to SCML2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SCML2 antibody [SCMAD14A] -
Description
Mouse monoclonal [SCMAD14A] to SCML2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, C terminal. (Human)
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Positive control
- HeLa Whole cell lysate, NIH3T3 whole cell lysate, and F2408 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab51506 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
SCMAD14A -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-SCML2 antibody [SCMAD14A] (ab51506)Anti-SCML2 antibody [SCMAD14A] (ab51506) at 1/200 dilution + HeLa whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 77.3 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Same results obtained using NIH3T3 whole cell lysate, and F2408 whole cell lysate.