VAMP8 / EDB overexpression 293T lysate (whole cell) (ab94103)
Overview
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Product name
VAMP8 / EDB overexpression 293T lysate (whole cell)
See all VAMP8/EDB lysates -
General notes
ab94103 is a 293T cell transfected lysate in which Human VAMP8 / EDB has been transiently over-expressed using a pCMV-VAMP8 / EDB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Tissue specificity: Platelets. Similarity: Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.
Images
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SDS-PAGE - VAMP8 / EDB overexpression 293T lysate (whole cell) (ab94103)ab94103 at 15µg/lane on an SDS-PAGE gel.
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Western blot - VAMP8 / EDB overexpression 293T lysate (whole cell) (ab94103)All lanes : Anti-VAMP8/EDB antibody (ab89158) at 1/500 dilution
Lane 1 :VAMP8 / EDB overexpression 293T lysate (whole cell) (ab94103)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
