Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Spermine synthase antibody [EPR9253] (ab151547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9253] to Spermine synthase
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Spermine synthase antibody [EPR9253]
    See all Spermine synthase primary antibodies

  • Description

    Rabbit monoclonal [EPR9253] to Spermine synthase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Spermine synthase aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, HeLa, Jurkat and K562 lysates; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Spermine synthase antibody [EPR9253] (ab151547)
    Western blot - Anti-Spermine synthase antibody [EPR9253] (ab151547)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab151547 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab151547 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab151547 and ab18058 (loading control to vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Spermine synthase antibody [EPR9253] (ab151547)
    Western blot - Anti-Spermine synthase antibody [EPR9253] (ab151547)
    All lanes : Anti-Spermine synthase antibody [EPR9253] (ab151547) at 1/1000 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : HeLa lysate
    Lane 3 : Jurkat lysate
    Lane 4 : K562 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 41 kDa

  • Flow Cytometry - Anti-Spermine synthase antibody [EPR9253] (ab151547)
    Flow Cytometry - Anti-Spermine synthase antibody [EPR9253] (ab151547)
    Flow cytometric analysis of permeabilized HeLa cells labeling Spermine synthase (red) with ab151547 at 1/10 dilution, compared to a nonspecific control antibody (green).
  • Anti-Spermine synthase antibody [EPR9253] (ab151547)
    Anti-Spermine synthase antibody [EPR9253] (ab151547)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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