Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 02, 2021

Anti-UMOD antibody [EPR20071] (ab207170)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20071] to UMOD
Suitable for: IHC-P, IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-UMOD antibody [EPR20071]
See all UMOD primary antibodies
Description

Rabbit monoclonal [EPR20071] to UMOD
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human urine and fetal kidney lysate; mouse and rat kidney lysates. IHC-P: Human, mouse and rat kidney tissues. IP: Human kidney lysate.
General notes
Previously labelled as Uromucoid.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20071
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on distal convoluted is observed [PMID: 23988501].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-UMOD antibody [EPR20071] (ab207170)

All lanes : Anti-UMOD antibody [EPR20071] (ab207170) at 1/10000 dilution

Lane 1 : Human urine
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 70 kDa
Observed band size: 110 kDa
why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID: 18310901).
Western blot - Anti-UMOD antibody [EPR20071] (ab207170)

All lanes : Anti-UMOD antibody [EPR20071] (ab207170) at 1/1000 dilution

Lane 1 : Mouse kidney lysate
Lane 2 : Rat kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 70 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The MW observed is consistent with the literature (PMID: 18310901).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on distal convoluted tubules of mouse kidney is observed [PMID: 23988501].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] (ab207170)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Strong cytoplasmic staining on distal convoluted tubules of rat kidney is observed [PMID: 23988501].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

UMOD was immunoprecipitated from 0.35 mg of human kidney lysate with ab207170 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Human kidney lysate 10µg (Input).

Lane 2: ab207170 IP in human kidney lysate.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207170 in human kidney lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 0.5 second.
Immunoprecipitation - Anti-UMOD antibody [EPR20071] (ab207170)

UMOD was immunoprecipitated from 0.35 mg of mouse kidney lysate with ab207170 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Human kidney lysate 10µg (Input).

Lane 2: ab207170 IP in human kidney lysate.

Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207170 in human kidney lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 0.5 second.
Anti-UMOD antibody [EPR20071] (ab207170)

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