Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UMOD antibody [EPR22887-217] (ab255825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22887-217] to UMOD
  • Suitable for: WB, IP, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-UMOD antibody [EPR22887-217]
    See all UMOD primary antibodies

  • Description

    Rabbit monoclonal [EPR22887-217] to UMOD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Rat urine, Mouse kidney and Mouse urine lysates. IHC-P: Rat kidney and Mouse kidney tissues. IP: Mouse urine. IHC-Fr: Mouse kidney tissue; rat kidney tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22887-217

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UMOD antibody [EPR22887-217] (ab255825)
    Western blot - Anti-UMOD antibody [EPR22887-217] (ab255825)
    All lanes : Anti-UMOD antibody [EPR22887-217] (ab255825) at 1/1000 dilution

    Lane 1 : Rat urine
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse urine

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    UMOD is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 24097801).

    Exposure time: Lane 1:10 seconds; Lanes 2-3:3 minutes.

    Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)
    Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling UMOD with Ab255825 at 1/100 (5.00 µg/ml) dilution followed by ab150077 AlexaFluora 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Positive staining on the thick ascending limb of the loop of Henle (TALH) in rat kidney (PMID: 28781372) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)
    Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling UMOD with ab255825 at 1/100 (5.00 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    Positive staining on the thick ascending limb of the loop of Henle (TALH) in mouse kidney (PMID: 28781372) is observed.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

  • Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (ab255825)
    Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (ab255825)

    UMOD was immunoprecipitated from 0.35 mg Mouse urine with ab255825 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255825 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse urine 10µg

    Lane 2: ab255825 IP in Mouse urine

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255825 in Mouse urine.

    Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

    Exposure time: 50 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UMOD with ab255825 at 1/100 dilution (5.0 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the thick ascending limb of the loop of Henle (TALH) in mouse kidney (PMID: 14569098) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling UMOD with ab255825 at 1/100 dilution (5.0 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the thick ascending limb of the loop of Henle (TALH) in rat kidney (PMID: 14569098) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Anti-UMOD antibody [EPR22887-217] (ab255825)
    Anti-UMOD antibody [EPR22887-217] (ab255825)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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