Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-UMOD antibody [EPR22887-217] (ab255825)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22887-217] to UMOD
Suitable for: WB, IP, IHC-Fr, IHC-P
Reacts with: Mouse, Rat

Product name

Anti-UMOD antibody [EPR22887-217]
See all UMOD primary antibodies
Description

Rabbit monoclonal [EPR22887-217] to UMOD
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Rat urine, Mouse kidney and Mouse urine lysates. IHC-P: Rat kidney and Mouse kidney tissues. IP: Mouse urine. IHC-Fr: Mouse kidney tissue; rat kidney tissue.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22887-217
Isotype

IgG
Research areas

Western blot - Anti-UMOD antibody [EPR22887-217] (ab255825)

All lanes : Anti-UMOD antibody [EPR22887-217] (ab255825) at 1/1000 dilution

Lane 1 : Rat urine
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse urine

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 71 kDa
Observed band size: 100 kDa
why is the actual band size different from the predicted?

UMOD is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 24097801).

Exposure time: Lane 1:10 seconds; Lanes 2-3:3 minutes.

Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling UMOD with Ab255825 at 1/100 (5.00 µg/ml) dilution followed by ab150077 AlexaFluora 488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Positive staining on the thick ascending limb of the loop of Henle (TALH) in rat kidney (PMID: 28781372) is observed.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

Immunohistochemical analysis of 4% PFA fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling UMOD with ab255825 at 1/100 (5.00 µg/ml) dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Positive staining on the thick ascending limb of the loop of Henle (TALH) in mouse kidney (PMID: 28781372) is observed.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-UMOD antibody [EPR22887-217] (ab255825)

UMOD was immunoprecipitated from 0.35 mg Mouse urine with ab255825 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255825 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Mouse urine 10µg

Lane 2: ab255825 IP in Mouse urine

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255825 in Mouse urine.

Blocking and dilution buffer and concentration/ 5% NFDM/TBST.

Exposure time: 50 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling UMOD with ab255825 at 1/100 dilution (5.0 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the thick ascending limb of the loop of Henle (TALH) in mouse kidney (PMID: 14569098) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR22887-217] (ab255825)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling UMOD with ab255825 at 1/100 dilution (5.0 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the thick ascending limb of the loop of Henle (TALH) in rat kidney (PMID: 14569098) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Secondary antibody only control/ Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Anti-UMOD antibody [EPR22887-217] (ab255825)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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