Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6239(2)] to Src (phospho Y418) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free
    See all Src primary antibodies

  • Description

    Rabbit monoclonal [EPR6239(2)] to Src (phospho Y418) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248506 is the carrier-free version of ab133460 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248506 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution (purified)

    Lane 1 : Untreated C6 (rat glioma) whole cell lysate
    Lane 2 : C6 (rat glioma) treated with pervanadate whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa



    This data was developed using ab133460, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution (purified)

    Lane 1 : HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate
    Lane 2 : Untreated HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa



    This data was developed using ab133460, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma) treated with pervanadate whole cell lysate
    Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 60 kDa



    This data was developed using ab133460, the same antibody clone in a different buffer formulation.

    Purified format. Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    Western blot - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution (undiluted)

    Lane 1 : A431 cell lysate, untreated
    Lane 2 : A431 cell lysate, treated with Pervanadate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 60 kDa



    This data was developed using ab133460, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    OI-RD Scanning - Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    This data was developed using ab133460, the same antibody clone in a different buffer formulation.Undiluted format. Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)
    Anti-Src (phospho Y418) antibody [EPR6239(2)] - BSA and Azide free (ab248506)

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