Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALPK1 antibody [EPR20911-169] (ab236626)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20911-169] to ALPK1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ALPK1 antibody [EPR20911-169]
    See all ALPK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20911-169] to ALPK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild type 293T, A549 lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20911-169

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626)
    Western blot - Anti-ALPK1 antibody [EPR20911-169] (ab236626)
    All lanes : Anti-ALPK1 antibody [EPR20911-169] (ab236626) at 1/500 dilution

    Lane 1 : ALPK1 knockout 293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg
    Lane 2 : Wild-type 293T whole cell lysate at 10 µg
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary
    All lanes : Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 139 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The image was kindly provided by the collaborator.

    ab236626 Anti-ALPK1 antibody [EPR20911-169] was shown to react with ALPK1 in 293T cells in Western blot. Loss of signal was observed when ALPK1 knockout sample was used. Wild-type and ALPK1 knockout samples were subjected to SDS-PAGE. ab236626 was incubated 1 hour at room temperature at 1/1000 dilution . Blots were developed with Donkey Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/5000 dilution for 1 hour at room temperature before imaging.  Anti-beta Tubulin was used as loading control.

    Exposure time: 5 minutes

     

  • Anti-ALPK1 antibody [EPR20911-169] (ab236626)
    Anti-ALPK1 antibody [EPR20911-169] (ab236626)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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