Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)
![Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.com/ps/products/185/ab185235/Images/ab185235-218609-anti-rrp4-antibody-epr13606b-c-terminal-western-blot.jpg "Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13606(B)] to RRP4 - C-terminal
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-RRP4 antibody [EPR13606(B)] - C-terminal
See all RRP4 primary antibodies -
Description
Rabbit monoclonal [EPR13606(B)] to RRP4 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, HeLa, 293 and Jurkat cell lysates; Human stomach tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13606(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)All lanes : Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.com/ps/products/185/ab185235/Images/ab185235-218608-anti-rrp4-antibody-epr13606b-c-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RRP4 with ab185235 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.com/ps/products/185/ab185235/Images/ab185235-218607-anti-rrp4-antibody-epr13606b-c-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)Flow cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling RRP4 with ab185235 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)](https://www.abcam.com/ps/products/185/ab185235/Images/ab185235-4-benefits-of-recombinant-antibodies.png)
Anti-RRP4 antibody [EPR13606(B)] - C-terminal (ab185235)
