Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13605] to RRP4 - N-terminal
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RRP4 antibody [EPR13605] - N-terminal
    See all RRP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13605] to RRP4 - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa and 293 cell lysates; Human kidney tissue; HepG2 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)
    Immunoprecipitation - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)

    Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab181211 at 1/50 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)
    Immunocytochemistry/ Immunofluorescence - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)

    Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling RRP4 with ab181211 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RRP4 with ab181211 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)
    Western blot - Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)
    All lanes : Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211) at 1/50000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 33 kDa

  • Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)
    Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-RRP4 antibody [EPR13605] - N-terminal (ab181211)

© 2021 Astana Biomed Group LLP. All rights reserved.