Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)
![Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)](https://www.abcam.com/ps/products/223/ab223540/Images/ab223540-5-ab207170267038ip1.jpg "Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20071] to UMOD - BSA and Azide free
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-UMOD antibody [EPR20071] - BSA and Azide free
See all UMOD primary antibodies -
Description
Rabbit monoclonal [EPR20071] to UMOD - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human urine and fetal kidney lysate; mouse and rat kidney lysates. IHC-P: Human, mouse and rat kidney tissues. IP: Human kidney lysate.
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General notes
ab223540 is the carrier-free version of ab207170 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab223540 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labelled as Uromucoid.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20071 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)
UMOD was immunoprecipitated from 0.35 mg of mouse kidney lysate with ab207170 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human kidney lysate 10µg (Input).
Lane 2: ab207170 IP in human kidney lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207170 in human kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 0.5 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207170).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)](https://www.abcam.com/ps/products/223/ab223540/Images/ab223540-4-ab207170266926207170IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Strong cytoplasmic staining on distal convoluted tubules of mouse kidney is observed [PMID: 23988501].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207170).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)](https://www.abcam.com/ps/products/223/ab223540/Images/ab223540-3-ab207170266925207170IHCc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Strong cytoplasmic staining on distal convoluted tubules of rat kidney is observed [PMID: 23988501].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207170).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)](https://www.abcam.com/ps/products/223/ab223540/Images/ab223540-2-ab207170266923207170IP.jpg)
Immunoprecipitation - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)UMOD was immunoprecipitated from 0.35 mg of human kidney lysate with ab207170 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207170 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Human kidney lysate 10µg (Input).
Lane 2: ab207170 IP in human kidney lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207170 in human kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 0.5 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207170).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)](https://www.abcam.com/ps/products/223/ab223540/Images/ab223540-282794-ab207170IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)This IHC data was generated using the same anti-UMOD antibody clone [EPR20071] in a different buffer formulation (cat# ab207170).
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling UMOD with ab207170 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Strong cytoplasmic staining on distal convoluted is observed [PMID: 23988501].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)](https://www.abcam.com/ps/products/223/ab223540/Images/ab223540-6-benefits-of-recombinant-antibodies.png)
Anti-UMOD antibody [EPR20071] - BSA and Azide free (ab223540)
