Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

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Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9)
Suitable for: WB, Dot blot
Reacts with: Mouse, Rat, Human

Product name

Anti-Synapsin I (phospho S9) antibody [EP2162Y]
See all Synapsin I primary antibodies
Description

Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Synapsin I (phospho S9). The exact sequence is proprietary.
Positive control
- WB: Human, mouse and rat brain tissue lysates, untreated or treated with AP.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP2162Y
Isotype

IgG
Research areas

Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

Lanes 1 & 3 : Human brain whole cell lysate - untreated
Lanes 2 & 4 : Human brain whole cell lysate - treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 77 kDa
why is the actual band size different from the predicted?

Exposure time:

Lanes 1 and 2: 1 second.

Lanes 3 and 4: 15 seconds.

Blocking and dilution buffer:

Lanes 1 and 2: 5% NFDM/TBST.

Lanes 3 and 4: 2% BSA/TBST.
Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution

Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution

Lane 1 : Mouse brain whole cell lysate - untreated
Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution

Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and dilution buffer: 2% BSA/TBST.
Western blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

Lane 1 : human brain tissue lysates, untreated
Lane 2 : human brain tissue lysates, treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa
Dot Blot - Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)

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