Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9)
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Synapsin I (phospho S9) antibody [EP2162Y]
See all Synapsin I primary antibodies -
Description
Rabbit monoclonal [EP2162Y] to Synapsin I (phospho S9) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Synapsin I (phospho S9). The exact sequence is proprietary.
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Positive control
- WB: Human, mouse and rat brain tissue lysates, untreated or treated with AP.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2162Y -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution
Lanes 1 & 3 : Human brain whole cell lysate - untreated
Lanes 2 & 4 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?Exposure time:
Lanes 1 and 2: 1 second.
Lanes 3 and 4: 15 seconds.
Blocking and dilution buffer:
Lanes 1 and 2: 5% NFDM/TBST.
Lanes 3 and 4: 2% BSA/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/5000 dilution
Lane 1 : Human brain whole cell lysate - untreated
Lane 2 : Human brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/200 dilution
Lane 1 : Mouse brain whole cell lysate - untreated
Lane 2 : Mouse brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260) at 1/1000 dilution
Lane 1 : Rat brain whole cell lysate - untreated
Lane 2 : Rat brain whole cell lysate - treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 74 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and dilution buffer: 2% BSA/TBST.
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All lanes : Anti-Synapsin I (phospho S9) antibody [EP2162Y] (ab76260)
Lane 1 : human brain tissue lysates, untreated
Lane 2 : human brain tissue lysates, treated with AP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa
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Dot blot analysis of Synapsin I (pS9) phospho peptide (lane 1) and Synapsin I non-phospho peptide (lane 2) labelling Synapsin I (pS9) with ab76260 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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