FITC Anti-MHC Class II antibody [M5/114] (ab239229)
![FITC Anti-MHC Class II antibody [M5/114] (ab239229)](https://www.abcam.com/ps/products/239/ab239229/Images/ab239229-343286-mhcIIantibodym5114fitc-flowcytometry.jpg "FITC Anti-MHC Class II antibody [M5/114] (ab239229)")
Key features and details
- FITC Rat monoclonal [M5/114] to MHC Class II
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-MHC Class II antibody [M5/114]
See all MHC Class II primary antibodies -
Description
FITC Rat monoclonal [M5/114] to MHC Class II -
Host species
Rat -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
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Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Tissue, cells or virus corresponding to Mouse MHC Class II. Activated C57BL/6 mouse spleen cells
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Positive control
- Flow cyt: Murine splenocytes.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. -
Clonality
Monoclonal -
Clone number
M5/114 -
Isotype
IgG2b -
Research areas
Images
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Flow Cytometry - FITC Anti-MHC Class II antibody [M5/114] (ab239229)
Surface staining of MHCII on murine splenocytes with ab239229 at 4 μg/ml in flow cytometric analysis.